Cat#:RANKL-100HRP;Product Name:Recombinant Human RANKL / TNFSF11 Protein;Synonym:RANKL,TNFSF11,CD254,OPGL,hRANKL2;Background:The full name of RANKL gene is receptor activator of nuclear factor-κB ligand gene. This gene, also known as TNFSF11 gene, encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. RANKL has been identified to affect the immune system and control bone regeneration and remodeling.;Description:Recombinant human RANKL protein was produced in Human cells. A DNA sequence encoding the human RANKL was expressed with the Fc Tag at the N-terminus. The RANKL human recombinant protein was purified by our unique purification techniques.;Source:Human Cells;Predicted N Terminal:Ser;AA Sequence:Gly 63-Asp 244;Molecular Characterization:The recombinant human RANKL protein has a predicted MW of 47.9 kDa containing 431aa. The apparent MW of the human RANKL protein is approximately 56 kDa in SDS-PAGE under reducing conditions due to glycosylation.;Purity:Greater than 85% by SDS PAGE;Endotoxin:< 1.0 EU per μg of the recombinant human rankl protein determined by the LAL method;Bioactivity:The ED50 is 1.5-7.5ng/mL;Formulation:RANKL human protein was lyophilized from sterile PBS, pH 7.4.;Stability:Recombinant human RANKL Protein is stable for up to 1 year from date of receipt at -80℃;Host Species:Human;Storage:Store recombinant human RANKL protein under sterile conditions at -20℃ to -80℃. It is recommended that the human rankl protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.;Usage:For Research Use Only!;References:Leibbrandt A, et al. (2008) RANK/RANKL: regulators of immune responses and bone physiology. Ann N Y Acad Sci. 1143: 123-50.;