Cat#:RP-1294HL;Product Name:Recombinant Human RANKL / OPGL / TNFSF11 / CD254 Protein(Fc Tag);Synonym:CD254,hRANKL2,ODF,OPGL,OPTB2,RANKL,sOdf,TRANCE;Description:A DNA sequence encoding the human RANKL/TNFSF11 isoform 2 (O14788-2) (Gly 63-Asp 244) was fused with the Fc region of human IgG1 at the N-terminus. Recombinant human RANKL protein was produced in Human cells. The RANKL human recombinant protein was purified by our unique chromatographic techniques.;Source:Human Cells;Predicted N Terminal:Glu 20;Molecular Characterization:The recombinant human RANKL/TNFSF11/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 443 amino acids and has a calculated molecular mass of 48.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFSF11/Fc monomer is approximately 50-5;Purity:Greater than 86 % as determined by SDS-PAGE;Endotoxin:< 1.0 EU per μg of the human RANKL protein as determined by the LAL method;Bioactivity:The bioactivity of recombinant human RANKL protein was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 0.1-0.4 μg/mL.;Formulation:Recombinant human RANKL protein was lyophilized from sterile PBS, pH 7.4;Stability:Recombinant human RANKL proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Lyophilized recombinant human RANKL protein is stable at 4-8℃ for 2-4 weeks. Upon reconstitution, Recombinant human RANKL protein should be stored at 4°C between 2-7 days and for future use below -20°C. Avoid freeze-thaw cycles.;References:Takayanagi H, et al. (2002) Signaling crosstalk between RANKL and interferons in osteoclast differentiation. Arthritis Res. 4 Suppl 3: S227-32.;