Cat#:RP-4457H;Product Name:Recombinant Human Myelin Basic Protein / MBP Protein;Synonym:Myelin basic protein, MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein.;Background:MBP, the full name is myelin basic protein. Gene ID: 4155. The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products.;Description:Recombinant Human MBP Protein expressed in E. coli is a single polypeptide chain containing 220 amino acids (1-197) and having a molecular weight of 23.9kDa. MBP is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MGSSHHHHHH SSGLVPRGSH MGSMGNHAGK RELNAEKAST NSETNRGESE KKRNLGELSR TTSEDNEVFG EADANQNNGT SSQDTAVTDS KRTADPKNAW QDAHPADPGS RPHLIRLFSR DAPGREDNTF KDRPSESDEL QTIQEDSAAT SESLDVMASQ KRPSQRHGSK YLATASTMDH ARHGFLPRHR DTGILDSIGR FFGGDRGAPK RGSGKVSSEE.;Purity:Greater than 85% as determined by SDS-PAGE.;Formulation:The MBP solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Store recombinant human MBP protein at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.;Usage:For Lab Research Use Only;References:Isoaspartic acid is present at specific sites in myelin basic protein from multiple sclerosis patients: could this represent a trigger for disease onset? Friedrich MG, et al. Acta Neuropathol Commun, 2016 Aug 12. PMID 27519525;