Cat#: | RP-3207H |
Product Name: | Recombinant Human FGF1 Protein, Sf9 |
Synonym: | HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a. |
Description: | Recombinant human Fibroblast Growth Factor-1 Protein / FGF1 protein produced in Sf9 insect cells is a single, glycosylated, polypeptide chain containing 140 amino acids and having a molecular mass of 15803 Dalton. The recombinant human FGF1 protein is purified by our unique chromatographic techniques. |
Source: | Baculovirus. |
AA Sequence: | The sequence of the first five N-terminal amino acids as determined and was found to be Met-Phe-Asn-Leu-Pro. |
Purity: | Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Bioactivity: | The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <10 ng/ml, corresponding to a specific activity of 100,000IU/mg. |
Formulation: | The sterile human FGF1 protein solution (1.8mg/ml) contains 20mM Tris HCl pH=7.9, 100mM KCl, 0.2mM DTT and 20% glycerol. |
Stability: | Recombinant Human FGF1 Proteins are stable for up to 1 year from date of receipt at -70℃ |
Storage: | Recombinant human Fibroblast Growth Factor acidic / FGF1 protein although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |