Cat#:RP-2249H;Product Name:Recombinant Human BMP4 Protein, Active;Synonym:BMP4, bone morphogenetic protein 4; BMP-2B; BMP2B, DVR4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6; BMP-4; Bone morphogenetic protein 2B; OTTHUMP00000178992;Description: Recombinant human Bone Morphogenetic Protein 4 / BMP4 Protein produced in HEK cells is a glycosylated disulfide linked homodimer, having a total molecular weight of 34kDa. The recombinant human BMP4 protein is purified by our unique chromatographic techniques.;Source:HEK293;AA Sequence:GASHASLIPE TGKKKVAEIQ GHAGGRRSGQ SHELLRDFEATLLQMFGLRR RPQPSKSAVI PDYMRDLYRL QSGEEEEEQI HSTGLEYPER PASRANTVRS FHHEEHLENI PGTSENSAFR FLFNLSSIPE NEAISSAELR LFREQVDQGP DWERGFHRIN IYEVMKPPAE VVPGHLITRL LDTRLVHHNV TRWETFDVSP AVLRWTREKQ PNYGLAIEVT HLHQTRTHQG QHV;Molecular Characterization:34kDa;Purity:Greater than 95% as obsereved by SDS-PAGE.;Bioactivity:The specific activity of recombinant human BMP4 protein was determined by the dose dependent induction of alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line) and is typically 4.53ng/ml corresponding to a Specific Activity of 220,750.55IU/mg.;Formulation:The recombinant huamn BMP4 protein was lyophilized from 0.7mg/ml in 2xPBS+6% ethanol.;Stability:Recombinant human BMP4 Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized recombinant human BMP4 protein in sterile 4mM HCl containing 0.1% endotoxin-free recombinant HSA.;Storage:Lyophilized recombinant human BMP4 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant human BMP4 protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.;References:Oida S, Iimura T, Maruoka Y, Takeda K, Sasaki S (November 1995). "Cloning and sequence of bone morphogenetic protein 4 (BMP-4) from a human placental cDNA library". DNA Seq. 5 (5): 273–5.;