Cat#: | IGF1R-007RPH |
Product Name: | Recombinant Human IGF1R Protein(His & GST Tag) |
Synonym: | IGF1R; insulin like growth factor 1 receptor; IGFR; CD221; IGFIR; JTK13 |
Gene Introduction: | Gene ID: 3480. IGF1R, the full name is insulin like growth factor 1 receptor. This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] |
Description: | A DNA sequence encoding the human IGF1R (NP_000866.1) cytoplasmic domain (Met 954-Cys 1367) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
Source: | Baculovirus-Insect Cells |
Predicted N Terminal: | Met |
AA Sequence: | Met 954-Cys 1367 |
Molecular Characterization: | The recombinant human IGF1R (aa 954-1367) /GST chimera consists of 651 amino acids and has a calculated molecular mass of 74.6 KDa. |
Purity: | Greater than 85% by SDS-PAGE |
Endotoxin: | < 1.0 EU per μg of the human IGF1R protein as determined by the LAL method |
Bioactivity: | The specific activity of the human igf1r protein was determined to be 554 nmol/min/mg using Poly(Glu,Tyr) 4:1 as substrate |
Formulation: | The IFG1R human was supplied in sterile 20 mM Tris, 500 mM NaCl, 20 % glycerol, pH 7.4. |
Stability: | Recombinant Human IGF1R Proteins are stable for up to 1 year from date of receipt at -70℃ |
Host Species: | Human |
Storage: | Store the human IGF1R protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
References: | The Prognostic and Clinicopathological Significance of IGF-1R in NSCLC: a Meta-Analysis. Zhao J, et al. Cell Physiol Biochem, 2017. PMID 28946136 |