Cat#:RP-3035C;Product Name:Recombinant Canine CCL8 Protein;Synonym:C-C motif chemokine 8, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, MCP-2, Small-inducible cytokine A8, CCL8, MCP2.;Description:Recombinant Canine CCL8 Protein expressed in E.coli is a single, non-glycosylated, polypeptide chain containing 76 amino acids and having a molecular mass of 8.8kDa. The recombinant canine CCL8 protein is purified using our unique chromatographic techniques.;Source:E.coli;AA Sequence:QPDSVSIPIT CCFSMVKRKI PMQKLESYMR ITNSQCPQEA VIFKTKASRE ICADPKQKWV QDYMNHLDQK SQAQKP.;Purity:Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The biological activity of recombinant mouse CCL8 protein determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.;Formulation:Recombinant canine CCL8 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH7.4.;Stability:Recombinant Canine CCL8 Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized canine CCL8 protein in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant canine CCL8 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution canine CCL8 protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;